HPLC
HPLC (High-Performance Liquid Chromatography) is an analytical technique used to separate, identify, and quantify components in a mixture. It's particularly useful for analyzing complex samples.
How it works
- Injection: A small volume of the sample is injected into the HPLC system.
- Separation: The sample components are separated as they pass through a column filled with stationary phase material. Different components interact differently with the stationary phase, causing them to separate.
- Detection: The separated components are detected as they exit the column, often using a UV or mass spectrometer.
- Data Analysis: The detector's signal is converted into a chromatogram, which provides information about the components in the sample.
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